Agriculture / WORLD

Delaware method profiles plant proteins without first extracting cells

Researchers report a validated method that digests proteins in methanol-fixed plant tissue. They applied it to healthy and fungus-infected tobacco leaves.

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University of Delaware researchers validated a proteomics method that digests proteins inside methanol-fixed plant tissue instead of grinding tissue and isolating proteins first. The approach preserves pathology information within cells while allowing proteins to be identified and measured.

The team applied it to healthy and fungus-infected tobacco leaves, finding immune-related proteins in plant cell-to-cell communication vesicles and toxic proteins in vesicles from the fungal pathogen. The university says more than 15 campus laboratories have used the platform, which is licensed to a supplier. It may support plant-disease research; it is not a field-validated diagnostic test or treatment.

Data

Timeline

In-tissue proteomics method

Proteins were digested and measured within methanol-fixed plant tissue.

  1. · Occurred

    Method reported

    The Delaware team reported a validated plant-cell proteomics method.

Methodology

The method was demonstrated on healthy and fungus-infected tobacco leaves; the source does not report a field test or treatment.

Updated:

Key figures

Source-reported indicators

Proteins were digested and measured within methanol-fixed plant tissue.

Campus laboratories using the platform: lower bound
≥15 count
2026-10-13 · Observed
Methodology

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Updated:

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